前列腺特异性抗原检测与前列腺疾病相关性分析

Analysis the relativity between prostate specific Antigen and prostate disease

目的前列腺特异性抗原(PSA)是检测前列腺癌和鉴别前列腺增生与前列腺癌的重要指标,近年来前列腺特异性抗原相关指标游离PSA(fPSA)与总PSA(tPSA)及fPSA/tPSA的应用极大提高了前列腺癌的诊断率。由于tPSA、fPSA测定值的高低与测定方法密切相关,不同的方法会导致不同的结果。我们采用电化学发光免疫分析技术对其进行测定,从而找出血清中tPSA检测值在4～10ng/ml之间的前列腺增生和前列腺癌患者fPSA/tPSA比值的临界值,为临床诊断前列腺癌提供依据。方法采用Roche Elecsys E170电化学发光免疫检测仪,分别测定其tPSA、fPSA,计算fPSA/tPSA的比值。结果前列腺癌与前列腺增生相比fPSA/tPSA的比值明显减低,差异有统计学意义(P<0.01)。结论使用电化学发光免疫试剂时以<0.15作为fPSA/tPSA临界值,为前列腺癌早期发现、监测及预后提供可靠的诊断依据。

Objective Prostate Specific Antigen（PSA）is an important indicator of detecting prostate cancer,and also the important identification of benign prostatic hyperplasia and prostate cancer.In recent years,the application of free PSA（fPSA）,total PSA（tPSA）and fPSA /tPSA making the diagnosis of prostate cancer a great improvement.As the measured values of tPSA and fPSA is closely related to the determining methods,different methods lead to different results.For providing the basis for clinical diagnosis of prostate cancer,we determining by using the electrochemiluminescence immunoassay to find benign prostatic hyperplasia and prostate cancer patients whose value of tPSA in the serum is among 4~10 ng/ml,and then find out their thresholds of fPSA/tPSA.Methods By using the Roche ElecsyeE170 electrochemiluminescence immunoassay detector,measured the values of tPSA and fPSA,then calculated the ratios of fPSA /tPSA.Results Comparing the values of fPSA /tPSA between the patients with prostate cancer and the patients with benign prostatic hyperplasia,the former was lower significantly.And this difference had great significance in the diagnosis（P0.01）.Conclusion Regarded 0.15 as the threshold of fPSA/tPSA when using the electrochemiluminescence immunoassay kit in the detections.This approach provided a reliable basis for early detecting,monitoring and preventing the prostate cancer.