粒细胞集落刺激因子对供体造血干细胞移植物中树突状细胞的影响

Influence of granuiocyte colony-stimulating factor on dendritic cells in donor of allogeneic hematopoietic stem cell grafts

目的探讨粒细胞集落刺激因子（G—CSF）对正常异基因造血干细胞移植供者外周血与骨髓移植物中I型树突状细胞（DC1）、Ⅱ型树突状细胞（DC2）的数量及DC2／DC1比例的影响。方法以G-CSF每天10μg／kg动员5d后，以流式细胞术（FCM）检测11例G—CSF动员的异基因外周血造血干细胞移植物及20例G—CSF动员的异基因骨髓移植物中的DC1、DC2数量及DC2／DC1比例，并与8例正常供者动员前外周血及10例健康者动员前骨髓进行比较。结果动员前后骨髓DC2由14．37×10^6／L增至29．68×10^6／L（t=2．433，P=0．022），而骨髓DC1分别为13．77×10^6／L和18．88×10^6／L（t=0．625，P=0．541）；DC2／DC。比例在动员后为1．83±0．81，较动员前的1．12±0．32明显升高（t=2．685，P=0．013）。正常供者以G—CSF动员前、后移植物中外周血DC2数量分别为14．92×10^6／L和26．76×10^6／L（t=2．390，P=0．029），DC2／DC1比例分别为1．00±0．37和2．02±1．43（t=2．158，P=0．044），但外周血DC1分别为14．21×10^6／L和18．02×10^6／L（t=0．625，P=0．541）。结论移植前以G—CSF动员正常异基因干细胞移植供者，可选择性提高外周血及骨髓移植物中DC2的数量，而DC1数量无明显增加。

Objective To evaluate the influence of granulocyte colony-stimulating factor （G-CSF） on the quantity and ratio of type 1 dendritic cells （DC1） and type 2 dendritic cells （DC2） in normal allogeneic hematopoietic stem cell graft donor peripheral blood （PB） and bone marrow （BM）. Methods G-CSF treatment with 10μg/kg per day for 5 days, the quantity, ratio of DC1 and DC2 in G-CSF primed allogeneic peripheral blood stem cell graft （G-PBSC） were detected in 11 donors, 20 donors in G-CSF primed allogeneic bone marrow stem cell graft （G-BMSC）, 8 donors in unprimed PB, and 10 normal individuals in unprimed BM, respectively, by flow cytometry method. Results After treated by G-CSF, the mean BMDC2 was increased from 14.37×10^6/L to 29.68×10^6/L （ t = 2.433, P = 0.022） in control group, whereas BMDCI count did not change （13.77×10^6/L vs 18.88×10^6/L, t = 1.169, P = 0.251）. The ratio of DC2 to DC2 was significantly higher in G-CSF treated donor BM （1.83±0.81 vs 1.12±0.32, t = 2.658, P =0.013）. After G-CSF treatment of normal donor, the number of PBDC2 （14.92×10^6/L vs 26.76×10^6/L, t = 2.390, P = 0.029）, and the ratio of DC： to DC1 was increased （1.00±0.37 vs 2.02±1.43, t = 2.158, P = 0.044）, but the number of PBDC1 was similar （14.21×10^6/L vs 18.02×10^6/L, t = 0.625, P = 0.541）. Conclusion G-CSF treatment of normal allogeneic HSC donor selectively increase the number of PBDC2 and BMDC2, but do not change PBDC1 and BMDC1.