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粒细胞集落刺激因子对供体造血干细胞移植物中树突状细胞的影响

Influence of granuiocyte colony-stimulating factor on dendritic cells in donor of allogeneic hematopoietic stem cell grafts
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摘要 目的探讨粒细胞集落刺激因子(G—CSF)对正常异基因造血干细胞移植供者外周血与骨髓移植物中I型树突状细胞(DC1)、Ⅱ型树突状细胞(DC2)的数量及DC2/DC1比例的影响。方法以G-CSF每天10μg/kg动员5d后,以流式细胞术(FCM)检测11例G—CSF动员的异基因外周血造血干细胞移植物及20例G—CSF动员的异基因骨髓移植物中的DC1、DC2数量及DC2/DC1比例,并与8例正常供者动员前外周血及10例健康者动员前骨髓进行比较。结果动员前后骨髓DC2由14.37×10^6/L增至29.68×10^6/L(t=2.433,P=0.022),而骨髓DC1分别为13.77×10^6/L和18.88×10^6/L(t=0.625,P=0.541);DC2/DC。比例在动员后为1.83±0.81,较动员前的1.12±0.32明显升高(t=2.685,P=0.013)。正常供者以G—CSF动员前、后移植物中外周血DC2数量分别为14.92×10^6/L和26.76×10^6/L(t=2.390,P=0.029),DC2/DC1比例分别为1.00±0.37和2.02±1.43(t=2.158,P=0.044),但外周血DC1分别为14.21×10^6/L和18.02×10^6/L(t=0.625,P=0.541)。结论移植前以G—CSF动员正常异基因干细胞移植供者,可选择性提高外周血及骨髓移植物中DC2的数量,而DC1数量无明显增加。 Objective To evaluate the influence of granulocyte colony-stimulating factor (G-CSF) on the quantity and ratio of type 1 dendritic cells (DC1) and type 2 dendritic cells (DC2) in normal allogeneic hematopoietic stem cell graft donor peripheral blood (PB) and bone marrow (BM). Methods G-CSF treatment with 10μg/kg per day for 5 days, the quantity, ratio of DC1 and DC2 in G-CSF primed allogeneic peripheral blood stem cell graft (G-PBSC) were detected in 11 donors, 20 donors in G-CSF primed allogeneic bone marrow stem cell graft (G-BMSC), 8 donors in unprimed PB, and 10 normal individuals in unprimed BM, respectively, by flow cytometry method. Results After treated by G-CSF, the mean BMDC2 was increased from 14.37×10^6/L to 29.68×10^6/L ( t = 2.433, P = 0.022) in control group, whereas BMDCI count did not change (13.77×10^6/L vs 18.88×10^6/L, t = 1.169, P = 0.251). The ratio of DC2 to DC2 was significantly higher in G-CSF treated donor BM (1.83±0.81 vs 1.12±0.32, t = 2.658, P =0.013). After G-CSF treatment of normal donor, the number of PBDC2 (14.92×10^6/L vs 26.76×10^6/L, t = 2.390, P = 0.029), and the ratio of DC: to DC1 was increased (1.00±0.37 vs 2.02±1.43, t = 2.158, P = 0.044), but the number of PBDC1 was similar (14.21×10^6/L vs 18.02×10^6/L, t = 0.625, P = 0.541). Conclusion G-CSF treatment of normal allogeneic HSC donor selectively increase the number of PBDC2 and BMDC2, but do not change PBDC1 and BMDC1.
出处 《白血病.淋巴瘤》 CAS 2011年第7期415-417,共3页 Journal of Leukemia & Lymphoma
关键词 粒细胞集落刺激因子 树突细胞 异基因造血干细胞移植 急性移植物抗宿主病 Granulocyte colony-stimulating factor Dendritic cells Allogeneic hematopoietic stem cell transplantion Acute graft versus host disease
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参考文献8

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