蛇床子素对体外培养大鼠乳鼠心肌细胞钾通道的影响

Effect of osthole on potassium channels of cadiocytes in neonate rats in vitro

目的研究蛇床子素对体外培养大鼠乳鼠心肌细胞钾通道电流的影响。方法采用全细胞膜片钳技术,首先在乳鼠心肌细胞上诱导出内向整流钾电流和瞬时外向钾电流,然后观察蛇床子素对这2种钾电流的影响。结果蛇床子素以浓度依赖的方式快速可逆地抑制心肌细胞瞬时外向钾电流,抑制的半有效浓度为(101.1±5.2)μmol.L-1,蛇床子素不改变瞬时外向钾通道的激活动力学。蛇床子素对内向整流钾电流有一定的抑制作用,在200μmol.L-1的浓度下抑制(68.2±7.5)%的内向钾电流。结论蛇床子素能够抑制乳鼠心肌细胞钾通道电流,并呈浓度依赖性。

Objective To investigate the effect of osthole on potassium channels electric current of cadiocytes in neonate rats in vitro.Methods The transient outward potassium currents and inward-rectifying potassium currents of cadiocytes in neonate rats were recorded by using whole cell patch clamp technique.The effects of osthole on potassium currents were investigated.Results Transient outward potassium currents were inhibited by osthole in a concentration-dependent manner,and the inhibition was fast and reversible.The half-effective concentration of the inhibition was（101.1±5.2）μmol·L-1.Osthole showed no significant influence on the activation kinetics of transient outward potassium channel.Osthole showed depressant effect on inward-rectifying potassium currents,osthole reduced the inward-rectifying potassium currents by（68.2±7.5）% at the concentration of 200 μmol·L-1.Conclusion Osthole concentration-dependently decreased potassium currents of cadiocytes in neonate rats in vitro.