血涂片染色方法的改良

The modification of blood smear staining methods

目的:探索提高血涂片细胞形态和颜色以及持久性的制作方法。方法:实验从染色冲洗的环节进行改良,采用蒸馏水(纯净水)取代自来水。结果:血涂片用Wright-Giemsa染色、蒸馏水(纯净水)冲洗的方法在光镜下观察细胞结构较清晰,层次分明,不易褪色,保存和使用时间较长。结论:该方法提高了血涂片染色的质量。

Objective：To explore the blood smear staining method, which allows good cell shape and color. Methods：The staining method and washing step were reformed and tap-water was replaced by double distilled water. Results：Cell structure and distinct gradations were clearly observed under microscopy with Wright-Giemsa mixed staining and double distilled water washing. Conclusion. This method is not easy to fade and suitable for long-term use and preservation, improving the quality of blood smeas staining.