芹黄素纳米脂质载体制剂的制备及理化性质考察

Preparation and in vitro Characterization of Apigemin-loaded Nanostructured Lipid Carriers

目的:制备芹黄素纳米脂质载体制剂并进行质量评价。方法:采用高温乳化-低温固化法制备芹黄素纳米脂质载体制剂,并对其形态、粒径、表面电位、包封率、载药量、体外释药等特性进行考察。结果:得到的芹黄素纳米粒为类球形,粒径分布均匀,平均粒径为212.1 nm,Zeta电位为-14.65 mV,平均包封率为82.4%,平均载药量为0.97%,体外2 h累积释药30%,随后释药过程趋于缓慢。结论:所制备的芹黄素纳米脂质载体制剂性质稳定,有良好的应用前景。

Objective：To prepare Apigemin-loaded nanostructured lipid carriers（Api-NLCs）and evaluate their characteristics.Methods：Api-NLCs were prepared by the method of emulsion evaporation-solidification at low temperature.The physicochemical properties such as morphology,size distribution,zeta potential,entrapment efficiency,drug loading and drug release in vitro were evaluated.Results：The obtained nanoparticles were spherical under transmission electron microscope.The mean diameter was 212.1 nm,the zeta potential was-14.65 mV,the mean entrapment efficiency was 82.4% and the mean drug loading was 0.97%.The total drug release was 30% in 2 hours followed by a slow and sustained release in vitro.Conclusion：The optimal Api-NLCs show stable characteristics and broad prospects for application.