相溶解度法测定羟丙基-β-环糊精与欧前胡素和异欧前胡素的超分子包合常数

Determination of Supermolecular Inclusion Constants of Hydroxylpropyl-β-cylcodextrin with Imperatorin and Isoimperatorin by Phase Solubility Method

目的:利用相溶解度曲线法研究不同浓度的羟丙基-β-环糊精(HP-β-CD)与欧前胡素和异欧前胡素的超分子相互作用。方法:配制一系列浓度的HP-β-CD溶液,加入过量的欧前胡素或异欧前胡素,绘制相溶解度图。结果:相溶解度曲线为线性关系,包合模型为AL型。说明欧前胡素和异欧前胡素均与HP-β-CD以1∶1包合,其包合常数分别为214.4、587.2 L/mol。结论:HP-β-CD与欧前胡素和异欧前胡素形成的超分子化合物均有较好的增溶作用,且对异欧前胡素的增溶作用优于欧前胡素。

Objective：To study the supermolecular interaction between different concentrations of hydroxypropyl-β-cyclodextrin（HP-β-CD）and imperatorin（IM） and isoimperatorin（ISO）using phase solubility analysis.Methods：Different concentrations of hydroxypropyl-β-cyclodextrin were prepared,added overdose of imperatorin or isoimperatorin,made a phase solubility diagram.Results：The relationship of phase solubility was approximatively linear and the model of inclusion compound was AL and the inclusion ratio of IM,ISO versus HP-β-CD was 1∶1.The inclusion constants were 214.4,587.2 L/mol respectively.Conclusions：Supermolecular inclusion of HP-β-CD of imperatorin/isoimperatorin could evidently increase the solubility of imperatorin and isoimperatorin,the enhancing effect of isoimperatorin solubility is better than that of imperatorin.