桃果实贮藏期间病原真菌的ITS rDNA序列分析与鉴定

ITS rDNA Sequence Analysis and Identification of Pathogens for Postharvest Diseases of Peach Fruit

目的:对桃果实采后病原菌进行分离与鉴定。方法:采用转接法分离桃果实采后病原真菌,经形态学观察和核糖体rDNA ITS(Internal transcribed spacer)区序列分析,对相关病原菌进行分类鉴定。结果:从采后贮藏过程中发生病害的桃果实分离到2株病原菌,经鉴定分别为美澳型核果链核盘菌(Monilinia fructicola)和葡萄座腔菌(Botryosphaeria dothidea)。结论:通过对ITS rDNA序列的相关分析,对分离自采后贮藏果实中的病原真菌进行有效的分类鉴定。

Separation and identification of Peaches pathogens. Methods： Pathogenic fungi in Beijing Pinggu Peaches was oeparated by transconjuge method and identified by morphology and ITS rDNA sequence analysis. Results： Two pathogens were isolated from post-harvest diseases of peach fruit,and identified through correlation analysis： Monilinia fructicola and Botryosphaeria dothidea. Conclusion： The analysis of ITS rDNA sequence can be effective to classify and identify the pathogenic fungi from the fruit with postharvest diseases.