摘要
根据15条具有菌株群特异性的SRAP、ISSR、RAPD扩增产物DNA条带测序结果,设计64对SCAR引物,其中6对引物能成功扩增出目的条带,即有6条特异片段(A、C、E、G、H、J)被成功转化为SCAR标记。通过这6个SCAR标记的不同组合,将40个双孢蘑菇菌株分为9个类群和6个类群,与基于SRAP、ISSR、RAPD资料的聚类分析结果的组间距离值取13和16时的分类结果完全吻合。对A片段的SCAR-PCR检测结果是:可从40个双孢蘑菇菌株中鉴别出双孢蘑菇333号菌株(编号01)。
Fifteen strain or strain groups-specific DNA bands amplificated from SRAP,ISSR,RAPD PCR were selected to be sequenced. According to the sequence data,64 pairs of SCAR primer were designed for SCAR-PCR test. Six pairs of SCAR primer could be used to amplify 6 objective bands successfully. It indicated that these 6 specific fragments marked as A,C,E,G,H,J respectively,were converted into SCAR marker successfully,by their relevant SCAR primer pairs A1,C4,E5,G5,H2,J3 respectively. Through combination among A1,C4,E5,G5,J3 fragments,40 strains could be divided into 6 and 9 strain groups. This result is agreement completely with the result that 40 varieties were classified into 6 and 9 major variety groups at 16 and 13 of distance value in the genetic relationship dendrogram. Additionally,sample 333(No.01) could be identified by PCR with primer pair A1,directly.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2011年第4期194-202,共9页
Journal of Chinese Institute Of Food Science and Technology
基金
国家质量监督检验检疫总局科技项目(2007IK159)
关键词
双孢蘑菇
SCAR
菌株群鉴定
Agaricus bisporus (Lange) Imbach
SCAR
identification for strain-groups