ORAC法测定保健食品抗氧化能力的体内外实验对比分析

Comparative Analysis of Antioxidant Activity in vitro and in vivo of Health Foods by ORAC Assasy

目的:探讨ORAC(oxygen radical absorbance capacity)荧光法测定保健食品抗氧化能力的体内体外实验结果的相关性。方法:以VC和3种保健食品为样品,用ORAC荧光法作为检测方法,用抗氧化能力(即Trolox当量)作为定量依据,通过体内体外实验测定保健食品的抗氧化能力。结果:ORAC荧光法测定保健食品抗氧化能力体内体外的实验结果存在一定相关性。当体外检测样品抗氧化值折合小鼠灌胃剂量大于25μmol Trolox/kg时,用该样品连续灌胃小鼠30d,动物体内抗氧化实验结果呈现阳性。结论:通过ORAC荧光法体外实现测定保健食品抗氧化能力,能够推测用同样方法检测动物体内抗氧化实验的结果。

Objective： To explore the antioxidant activity of health foods in vivo and in vitro by ORAC（oxygen radical absorbance capacity） fluorometric assay and to carry out correlation analysis.Methods： The antioxidant activities of vitamin C and 3 health foods were evaluated in trolox equivalent antioxidant capacity.Results： A correlation between the in vivo and in vitro antioxidant activities of health foods was observed.The antioxidant activity of samples whose in vitro antioxidant activity was larger than the gavage dose of 25μmol Trolox/kg body weight for mice was determined as positive in mice after oral administration for 30 consecutive days.Conclusion： Under certain conditions,a correlation between the in vivo and in vitro antioxidant activities of health foods determined by ORAC exists,which can be used for speculating the antioxidant activity in vivo of heath foods by ORAC determination in vitro.