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二硫键对Enterocin A抗李斯特菌LIN3活性的影响 被引量：6

Effect of Disulfide Bond on Anti-Listeria innocua LIN3 Activity of Enterocin A
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摘要 依据Enterocin A的氨基酸序列,设计半胱氨酸替换突变体Enterocin A(C14S)和Enterocin A(C47W),通过大肠杆菌表达系统获得重组突变体。用还原剂β-巯基乙醇处理MBP-Enterocin A。采用琼脂扩散实验检测重组Enterocin A、突变体及还原产物的抗无害李斯特菌LIN3活性。结果表明:N末端融合部分不含有半胱氨酸的MBP-Enterocin A或His tag-Enterocin A均表现强抗菌活性;而N末端融合部分含有半胱氨酸的GST-Enterocin A,表现很弱的抗菌活性;半胱氨酸替换突变体及MBP-Enterocin A还原产物均不显示抗菌活性。实验结果证明二硫键为Enterocin A活性的重要影响因素。 Based on the amino acid sequence of Enterocin A,two cysteine substitution mutants,Enterocin A（C14S） and Enterocin A（C47W） were designed and obtained in E.coli expression systems.The agar diffusion assay was used to test the anti-Listeria innocua LIN3 activity of the mutants,recombinant Enterocin A and β-mercaptoethanol-reduced recombinant Enterocin A.Recombinant Enterocin A with His-tag at N-terminus revealed stronger antibacterial activity,while GST-Enterocin A revealed a significant decrease in antibacterial activity.The two mutants andβ-mercaptoethanol-reduced recombinant Enterocin A with destroyed disulfide bonds exhibit no detectable antibacterial activity.Therefore,disulfide bonds are essential for the antibacterial activity of Enterocin A.
作者 赵爱珍 徐兴然 韩文瑜
机构地区 西南大学药学院 吉林大学畜牧兽医学院
出处 《食品科学》 EI CAS CSCD 北大核心 2011年第15期182-185,共4页 Food Science
关键词 EnterocinA 二硫键 抗李斯特菌活性 Enterocin A disulfide bond anti-Listeria activity
分类号 TS201.3 [轻工技术与工程—食品科学]
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参考文献21

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二级参考文献52

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共引文献10

同被引文献99

  • 1柳峰松,王丽娜,唐婷,李伟.家蝇抗菌肽Diptericin基因的克隆与分析[J].昆虫学报,2009,52(10):1078-1082. 被引量：11
  • 2金小宝,许琴英,徐建华,朱家勇.家蝇幼虫天蚕素基因的克隆与序列分析[J].中国热带医学,2004,4(6):903-906. 被引量：15
  • 3耿华,安春菊,郝友进,李德森,杜荣骞.家蝇攻击素(Attacin)基因的克隆与表达[J].Acta Genetica Sinica,2004,31(12):1344-1350. 被引量：17
  • 4袁文,尹学琼,贺永宁,林强.壳聚糖羧基化的研究进展[J].化学试剂,2007,29(5):277-280. 被引量：5
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引证文献6

二级引证文献20

食品科学
2011年 第15期

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