西藏红景天对高原肺水肿大鼠肺组织影响

Effects of Rhodiola tibetica on lung tissue of rats with high altitude pulmonary edema

目的观察藏药红景天对高原肺水肿大鼠肺组织的影响及其低氧诱导因子-1a(HIF-1a)表达变化。方法将50只雄性SD大鼠随机分为5组,对照、生理盐水、红景天组(西安,海拔5m)、低氧组、红景天高原组(西藏那曲,海拔4500 m),光、电镜观察肺组织标本,免疫组化法检测肺组织HIF-1a表达,反转录聚合酶链式反应(RT-PCR)方法检测动物肺组织HIF-1a mRNA表达变化。结果低氧组肺组织显微和超微结构出现明显的高原肺水肿,红景天组高原肺水肿明显减轻;肺组织内未见HIF-1a蛋白表达;红景天高原组HIF-1a mRNA(0.463±0.069)表达明显高于对照组(0.231±0.037)和低氧组(0.219±0.047)(P<0.01)。结论藏药红景天可提高对大鼠肺组织HIF-1a mRNA表达,有利于减轻大鼠低氧性高原肺水肿。

Objective To observe the effects of Rhodiola tibetica（R.tibetica） on lung tissue of rats with high altitude pulmonary edema and the expression of hypoxia-inducible factor-1a（HIF-1a） in the rats.Methods Totally 50 male SD rats were randomly divided into 5 groups：control group,normal saline treatment group,R.tibetica acclimatization group at low altitude（in Xi＇an area with the altitude of 5 meters）,hypoxia group,and R.tibetica acclimatization group at high altitude（in Naqu area with the altitude of 4500 meters）.The changes of lung tissue of the rats were observed with light and electron microscope.HIF-1 expression in lung tissue was detected with immunohistochemical method and HIF-1a mRNA in lung tissue was determined with reverse transcriplase-PCR（RT-PCR）.Results Observations of microstructure and ultrastructure of lung tissues revealed obvious high altitude edema in hypoxia groups and the mitigated edema in R.tibetica acclimatization group was observed.The expression of HIF-1a mRNA of R.tibetica acclimatization group at high altitude was significantly higher than that of control group and hypoxia group（0.463±0.069 vs 0.231±0.037 and 0.219±0.047,P0.001）.Conclusion R.tibetica can increase the expression of HIF-1a mRNA in lung tissue and mitigate high altitude pulmonary edema in hypoxic rats.