不同温度下水稻胚乳AGPase各同工酶基因表达特征初步分析

A Preliminary Analysis of Gene Expression of Seven Isoforms of ADP-glucose Pyrophosphorylase(AGPase) in Rice Endosperm under Different Temperature Conditions

以特青和泰国香米为材料,利用人工气候箱设置高温(日平均温度33℃)和适温(日平均温度25℃)2个温度处理,结合实时荧光定量PCR技术分析比较了水稻淀粉合成关键酶腺嘌呤-葡萄糖焦磷酸化酶(AGPase)7个同工酶基因AGPS1、AGPS2a、AGPS2b、AGPL1、AGPL2、AGPL3及AGPL4的表达特征。结果表明,AGPS2b、AGPL2、AGPL3等3个同工酶基因表达较强,可能是水稻胚乳中AGPase基因表达的主要形式,其中AGPL2相对表达量最高;AGPS2b、AGPL2、AGPL3在2个品种中的相对表达量均为适温处理高于高温处理,在特青胚乳中各时期2种温度处理下的相对表达量总体高于泰国香米中的相对表达量。

Different temperature treatments（33℃ and 25℃ of daily mean temperature for high and normal temperature treatments,respectively） and the real-time fluorescence quantitative PCR（FQ-PCR） were used to analyze the expression patterns of seven isoforms（AGPS1,AGPS2a,AGPS2b,AGPL1,AGPL2,AGPL3 and AGPL4） of ADP-glucose pyrophosphorylase（AGPase） which was the key enzyme in starch synthesis metabolism in rice endosperm with two rice varieties Teqing and Thai Fragrant Rice as the materials.The results showed that the three isoforms of AGPS2b、AGPL2、AGPL3 had much higher expression amounts than the other four isoforms,thus they were thought to be the main expression patterns of AGPase in rice endersperm.The relative expression amounts of AGPL2 was the highest among all isoforms.The relative expression amounts of AGPS2b、AGPL2、AGPL3 were higher in the normal temperature treatment than in the high temperature treatment in both rice varieties.The relative expression amounts of the three enzyme genes at all tested milking stages in Teqing were higher than those in Thai Fragrant Rice under different temperature treatments.