乳腺癌患者血清胰岛素样生长因子-1及结合蛋白-3含量测定

Determination of serum insulin-like growth factor-1 and its binding protein-3 in patients with breast cancer

目的探讨胰岛素样生长因子-1(IGF-1)和胰岛素样生长因子结合蛋白-3(IGFBP-3)在乳腺癌中的表达和意义,并研究其与化疗疗效的关系。方法运用酶联免疫吸附法(ELISA)检测32例乳腺癌患者(A组),20例乳腺良性肿瘤患者(B组)和15例健康人(C组)血清中IGF-1和IGFBP-3的含量。结果 A组血清中IGF-1水平为(628.25±124.50)μg/L,B组血清中IGF-1水平为(435.46±116.86)μg/L,C组血清中IGF-1水平为(413.65±104.12)μg/L,A组明显高于B、C组且差异有统计学意义(P<0.05),但B组与C组之间差异无统计学意义(P>0.05)。A、B、C组血清中IGFBP3水平分别为(3596.12±1056.32)μg/L、(3856.12±1142.39)μg/L和(3912.65±1153.14)μg/L,3组之间差异无统计学意义(P>0.05)。15例化疗有效的患者,化疗2周期后IGF-1水平较化疗前明显降低,而IGFBP3水平呈高表达(P<0.05)。结论血清IGF-1和IGFBP-3含量变化有助于乳腺癌的辅助诊断和化疗疗效预测。

Objective To study the expression and significance of serum insulin-like growth factor-1（IGF-1） and insulin-like growth factor binding protein-3（IGFBP-3） level in breast cancer development,and the relationship between them and chemotherapy.Methods Serum IGF-1 and IGFBP-3 were determined by ELISA in 32 breast cancer patients（A group） ,20 benign breast disease（B group） and 15 healthy controls（C group） .Results Serum IGF-1 level in A group（628.25±124.50ug/L） ,was significantly higher than that in B group（435.46±116.86） μg/L and C group（413.65±104.12） μg/L（P0.05） ,but there was no significance between B group and C group（P0.05） .Serum IGFBP-3 level in A,B,C groups was（3596.12±1056.32） μg/L,（3856.12±1142.39） μg/L,（3912.65±1153.14） μg/L,respectively.There was no significant difference between them（P0.05） .In 15 patients obtaining response after 2 cycles chemotherapy serum IGF-1 level decreased significantly and IGFBP3 level increased significantly（P0.05） .Conclusions Serum IGF-1 and IGFBP3 level play an important role in the diagnosis of breast cancer and prediction of chemotherapy efficacy.