青岛地区腹泻患儿星状病毒感染的分子流行病学特征

A preliminary study of the molecular characteristics of astrovirus detected in Qingdao

目的研究青岛地区婴幼儿腹泻患者星状病毒流行病学特点和基因型别。方法收集青岛地区散发儿童病毒性腹泻患者粪便标本48份,经胶体金法、RT-PCR法分别检测轮状病毒和诺如病毒后,应用RT-PCR进行星状病毒检测,并对阳性标本进行测序,鉴定型别。结果 48份粪便标本中,4份检出星状病毒(8.3%),经序列分析鉴定为HastV-1型。结论青岛地区散发病毒性腹泻患儿存在星状病毒感染,型别为HastV-1。

Objective To study the pathogenic prevalence and genotypes of human astrovirus（HAtsV）in cases of children with viral diarrhea in Qingdao.Methods Forty-eight children with sporadic viral diarrhea in Qingdao were first screened for Rotavirus and Norwalk-like virus with Rotavirus Group A Diagnostic Kit（Collodial Gold Device）and reverse-transcription polymerase chain reaction（RT-PCR）,respectively.Samples were subjected to RT-PCR to detect astrovirus and positive PCR products were sequenced and classified.Results Among the 48 fecal specimens,a total of 4 tested positive（8.3%） and were identified as HAtsV-1 according to DNA sequence analysis.Conclusion Astrovirus infection was found in children with viral diarrhea in Qingdao and the genotype of astrovirus was identified as HAtsV-1.