摘要
目的:建立以高效液相色谱-质谱联用法测定人血浆中非索非那定浓度的方法。方法:以苯乙双胍为内标物,采用C18柱,流动相由乙腈及30 mmol/ml NH4Ac(含0.1%甲酸,0.01%三氟乙酸)组成,采用梯度洗脱程序,测定血浆中非索非那定浓度。结果:非索非那定在2.26~676.46 ng/ml线性关系良好(r=0.999 6,n=7),定量下限浓度为2.26 ng/ml,RSD为0.7%,高、中、低浓度测定的相对回收率分别为(109.9±1.7)%、(103.1±7.2)%和(101.5±13.4)%,日内RSD分别为2.1%、2.5%和9.8%,日间RSD分别为1.3%、5.7%和7.1%。结论:所建方法预处理简单﹑专属性强、灵敏度高,适合测定非索非那定的血药浓度。
Objective: To establish the LC-MS/MS method for the determination of fexofenadine in human plasma.Methods: Phenformin was used as internal standard.C18 column was used with the mobile phase of acetonitrile and 30 mmol/mlNH4Ac(containing 0.1% formic acid and 0.01% trifluoraceticacid).The concentration of fexofenadine in human plasma was determined by the method of LC-MS/MS with gradient elution.Results: A linearity was obtained from 2.26 to 676.46 ng/ml of fexofenadine in human plasma with a good correlation coefficient(r=0.999 6,n=7),with the quantitative lower limit was 2.26 ng/ml.RSD was 0.7%.The mean recoveries were(109.9±1.7)%,(103.1±7.2)% and(101.5±13.4)% respectively in the quality control samples of high,middle and low concentration.The RSD with in day and between day were 2.1%,2.5%,9.8%(n=5) and 1.3%,5.7%,7.1%(n=5),respectively.Conclusion: The method can be applied to determine the fexofenadine concentration in plasma.
出处
《中国医药导报》
CAS
2011年第22期28-30,共3页
China Medical Herald