百蕊草药材的指纹图谱研究

HPLC fingerprint of Thesium chinese Turcz.

目的采用高效液相色谱法建立来自14个不同产地的15批百蕊草样品的指纹图谱。方法甲醇加热回流提取1h获得百蕊草提取液,采用HPLC分析百蕊草的指纹图谱。色谱柱Inertsil OSD-SP(4.6 mm×150 mm,5μm);流动相甲醇-0.4%磷酸水溶液梯度洗脱;分析时间75 min;检测波长365 nm;柱温30℃;体积流量0.8 mL/min。结果建立了百蕊草的指纹图谱,5号山柰素峰为对照峰,确定了8个共有峰。结论该指纹图谱方法简单,重现性好,可作为百蕊草药材质量控制的重要方法。

AIM To establish chromatographic fingerprints of fifteen batches of Thesium chinese Turcz.from fourteen different habitats by HPLC.METHODS The extract of Thesium chinese Turcz.was obtained with methyl hydrate by thermal reflux for an hour.Analysis was performed on Inertsil OSD-SP column（4.6 mm×150 mm,5 μm） with gradient mobile phase of methanol-0.4% H3PO4.The fingerprint finished in 75 min,the detection wavelength was at 365 nm and the column temperature was 30 ℃ with the flow rate of 0.8 mL/min.RESULTS Establishing the fingerprints of Thesium chinese Turcz.,marker peak was kaempferide（No.5 peak）,eight common peaks were found in the HPLC fingerprints from different habitats.CONCLUSION The method is simple,accurate,with good reproducibility,and it can be used specifically for the quality control of Thesium Chinese Turcz.