HIV/AIDS患者CD4^+、CD8^+及WBC检测分析

Detection of CD4^+,CD8^+ and WBC from HIV/AIDS patients

目的分析HIV/AIDS患者外周血T辅助细胞(CD4+)、T抑制细胞(CD8+)和白细胞(WBC)检测结果。方法 CD4+和CD8+计数通过免疫荧光标记,采用流式细胞仪检测;白细胞(WBC)计数采用全自动细胞分析仪检测。结果 118例HIV/AIDS住院患者,WBC降低组(3.00±0.61)×109/L的CD4+和CD8+分别为(68.60±38.98)个/ul和(266.89±126.52)个/ul,WBC正常组(7.13±5.12)×109/L的CD4+和CD8+分别为(104.39±61.37)个/ul和(380.17±134.06)个/ul,两者差异有统计学意义(t1=5.78t,2=3.62t,3=5.35,P均<0.01);两组别CD4+/CD8+比值比较均呈有统计学意义(P<0.05)。结论 HIV/AIDS患者在发病过程中与CD4+、CD4+/CD8+、WBC有密切相关,建议HIV感染者尽早进行医学咨询并做CD4+、CD8+和血常规的检测,以了解病情的进展及进行抗病毒治疗。

Aim Analysis the relevance of peripheral T helper cell （CD4^＋）,T inhibited cell （CD8^＋）count and white blood cell （WBC） testing results of 118 cases of HIV/AIDS patients in our hospital. Methods CD4^＋ and CDS^＋ T iymphoeyte count are detected by flow cytometty,white blood cell（WBC） count is detected by cell analyzer. Results （1） In 118 eases of HIV / AIDS in hospitalized patients,CD4^＋ and CD8^＋ of WBC reduction group （3.00±0.61）×10^9/L are respectively （68.6±38.98）/ul and （266.89±126.52）/ul. CD4＋ and CD8＋ of WBC normal group （7.13±5.12）×10^9/L are respectively （104.39±61.37）/ul and （380.17±134.06）/ul. The both difference has statistics significance,see Table 1 （tl= 5.78,t2= 3.62,t3= 5.35 P〈0.01 ）. （2） CD4^＋values are divided into different group （see Table 2）. Each group comparison with CD4^＋/CD8^＋ ratio shows signlficandy correlation,the correlation coefficient is respectively 0.586,0.698 and 0.409 （P〈 0.05）. Conclusion It is closely related that HIV/AIDS patients in the disease process and the CD4^＋,CD8^＋,WBC. HIV infection are recommended medical advice as soon as possible,understand the progress of the disease and treat antiviral in time by CD4^＋, CD8^＋ and blood routine testing. HIV/AIDS patients in the course of treatment should be CD4^＋, CD8^＋ and blood routine testing for regular.