摘要
To perform the mechanism study of special association for vancomycin and D-Ala-D-Ala-containing peptides on the interface of solution and self-assemble monolayer,the binding between vancomycin and pentapeptide(Lys-Lys-Gly-D-Ala-D-Ala) was investigated by flow injection surface plasmon resonance(FI-SPR) and flow injection quartz crystal microbalance(FI-QCM).To facilitate the formation of a compact vancomycin adsorbates layer with a uniform surface orientation,vancomycin molecules were attached onto a preformed alkanethiol self-assembled monolayer.By optimizing the conditions for the binding between Lys-Lys-Gly-D-Ala-D-Ala and vancomycin on the assembled chip,the detecting limit of Lys-Lys-Gly-D-Ala-D-Ala was greatly improved(reaching 0.5×10-6 mol/L or 7.5×10-12 mol).The equilibrium constant of the association of Lys-Lys-Gly-D-Ala-D-Ala with vancomycin was also obtained(KAds=5.0×104 L/mol).
To perform the mechanism study of special association for vancomycin and D-Ala-D-Ala-containing peptides on the interface of solution and self-assemble monolayer, the binding between vancomycin and pentapeptide (Lys-Lys-Gly-D-Ala-D-Ala) was investigated by flow injection surface plasmon resonance (FI-SPR) and flow injection quartz crystal microbalance (FI-QCM). To facilitate the formation of a compact vancomycin adsorbates layer with a uniform surface orientation, vancomycin molecules were attached onto a preformed alkanethiol self-assembled monolayer. By optimizing the conditions for the binding between Lys-Lys-Gly-D-Ala-D-Ala and vancomycin on the assembled chip, the detecting limit of Lys-Lys-Gly-D-Ala-D-Ala was greatly improved (reaching 0.5 ×10^- 6 mol/L or 7.5 × 10^-12 mol). The equilibrium constant of the association of Lys-Lys-Gly-D-Ala-D-Ala with vancomycin was also obtained (KAds=5.0×10^4 L/tool).
基金
Projects(20773165,20876179) supported by the National Natural Science Foundation of China
Project(09JJ1002) supported by the Hunan Provincial Natural Science Foundation,China
Project(NCET-07-0865) for New Century Excellent Talents in Chinese University
Project(2007AA022006) supported by the National High Technology Research and Development Program of China