铍试剂Ⅱ催化荧光法测定痕量铁(Ⅲ)

CATALYTIC FLUORIMETRIC DETERMINATION OF TRACE IRON(Ⅲ) USING BERRYLLON Ⅱ

在pH为3．9的HAC／NaAc缓冲体系中，铍试剂Ⅱ被溴酸钾缓慢分解，铁（Ⅲ）对分解反应有催化作用，产物呈现荧光，其荧光峰为λex／λem＝317／423（nm），基于此建立了催化荧光测定痕量铁（Ⅲ）的最佳条件．铁（Ⅲ）的量在0．0－10．0μg／25mL范围内与荧光强度呈线性关系，回归方程X（μg／25mL）＝0．166ΔF－0．545，检测限为0．68μg／L，方法应用于自来水中铁（Ⅲ）的测定。

A new kinetic method has been described for the determination of trace lron (Ⅲ) in pH 3 .9HAc/NaAc medium, based on its catalytic action on the oxidation of Berryllon Ⅱ by potassium bromate.The λex/λem of the oxidation product is 317/423 (nm). The optimum conditions for determination of lron(Ⅲ) has been studied. The liner ranges of lron (Ⅲ) is O ~ 10.0 μg/25 mL,, the regression equation isX(μg/25 mL) = 0. 166Δ F - 0. 545, and the detection limit is 0. 68 μg/L. The method has been successfully applied to the determination of lron (Ⅲ) in tap water.