高效液相色谱法测定满金止咳片中芦丁含量

Study on Quality Standards for Manjinzhike Tablets

目的:建立满金止咳片中芦丁含量的测定方法。方法:运用高效液相色谱法(HPLC)对芦丁含量进行测定,色谱柱:Dikma C18(5um,150mm×4.6mm),流动相:甲醇-1%冰醋酸(35∶65)为流动相,检测波长:360nm,流速1.0mL/min,柱温:室温。结果:芦丁的量在41.3-206.5μg/ml范围内,浓度同峰面积呈良好的线形关系。平均加样回收率为99.22%,RSD=1.99%。结论:所建立的方法可靠,可用于满金止咳片的质量控制。

Objective：Established method for determination in Rutin of Manjinzhike Tablets.Methods：Using high performance liquid chromatography（HPLC） and determining on Rutin,column：Dikma C18（5um,150mm × 4.6mm） ,mobile phase：methanol-1% Ethanic acid（35：65） ,detection wavelength：360nm,flow rate 1.0mL/min,column temperature：room temperature.Results：The amount of Rutin in 41.3-206.5μg/ml within the peak area with concentration showed a good linear relationship.The average recovery was 99.22%,RSD = 1.99%.Conclusion：The method is reliable and can be used for quality control of Manjinzhike Tablets.