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一种新型的截短型转化生长因子-βⅡ型受体的构建 被引量：1

The development of a novel truncated typeⅡtransforming growth factor-β
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摘要 目的设计并构建一种新型的截短型转化生长因子-βⅡ受体(tTGF-βRⅡ)。方法 RT-PCR方法扩增TGF-βⅠ的CDS序列、tTGF-βRⅡ,并将其分别克隆至pGBKT7和pGADT7载体中。利用酵母双杂交和GST-pulldown试验检测tTGF-βRⅡ与TGF-βⅠ间的相互作用。人工合成该tTGF-βRⅡ多肽。结果成功地设计并克隆了一种新的tTGF-βRⅡ,其可以与配体TGF-βⅠ结合。结论制备的tTGF-βRⅡ可以作为TGF-βRⅡ的拮抗剂,竞争性的与TGF-βⅠ结合,为瘢痕治疗奠定了基础。 Purpose To design and clone a novel truncated typeⅡtransforming growth factor-β（tTGF-βRⅡ）.Methods Using RT-PCR to amplify the coding sequence of TGF-βⅠand tTGF-βRⅡ and cloned into vector pGBKT7 and pGADT7.Using yeast two-hybrid assay and GST-pull down assay to detect the interaction between TGF-βⅠand tTGF-βRⅡ.The tTGF-βRⅡpolypeptide was synthesized and used in the keloid fibroblasts.Results We design and clone a novel tTGF-βRⅡsuccessfully,which can bind to its ligand TGF-βⅠ.Conclusion This kind of tTGF-βRⅡ can work as a novel antagon of TGF-βRⅡ to bind with TGF-βⅠ,which may provide a new strategy for scar therapy.
作者 吴丹 刘洁婷 李洪志 赵冰海 袁晓环 初彦辉
机构地区 牡丹江医学院黑龙江省抗纤维化生物治疗重点实验室
出处 《中国生化药物杂志》 CAS CSCD 北大核心 2011年第4期287-290,共4页 Chinese Journal of Biochemical Pharmaceutics
基金 黑龙江省教育厅重大项目(12511z028)
关键词 截短型转化生长因子-βⅡ受体 转化生长因子Β 酵母双杂交 truncated typeⅡtransforming growth factor-β transforming growth factor-β yeast two-hybrid assay
分类号 Q78 [生物学—分子生物学]
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参考文献11

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二级参考文献17

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共引文献1

同被引文献15

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中国生化药物杂志
2011年 第4期

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