广东兰花两种主要病毒的检测

Detection of two major viruses in orchids in Guangdong

采用双抗夹心酶联免疫吸附法(DAS-ELISA)对44个兰花病样分别检测建兰花叶病毒(Cymbidium mosaic virus,CyMV)和齿兰环斑病毒(Odontoglossum ringspot virus,ORSV),结果显示,有59.1%的兰花检出CyMV,36.4%的兰花检出ORSV,22.7%的兰花复合感染了CyMV和ORSV。采用一步逆转录聚合酶链式反应(RT-PCR)对24个兰花病样进行检测,结果有83.3%的兰花检出CyMV,75%的兰花检出ORSV,66.7%的兰花复合感染了CyMV和ORSV,可见RT-PCR检测灵敏度高于ELISA。

Cymbidium mosaic virus（CyMV） and Odontoglossum ringspot virus （ORSV） were evaluated by DAS-ELISA respectively in 44 diseased orchid samples collected from Guangdong province, the resohs showed that CyMV and ORSV were detected in 59.1% and 36.4% respectively, mixed infections with CyMV and ORSV were detected in 22.7% of the 44 diseased orchid samples. CyMV and ORSV were evaluated by one step RT-PCR respectively in 24 diseased orchid samples collected from Guangdong province, the results showed that CyMV and ORSV were detected in 83.3% and 75% respectively, mixed infections with CyMV and ORSV were detected in 66.7% of the 24 diseased orchid samples. These results suggested that the sensitivity of RT-PCR method is higher than that of DAS-ELISA method.