马铃薯晚疫病抗性相关基因定位及QTL位点分析

Mapping of gene related to late blight resistance in potato and QTL analysis

【目的】寻找与马铃薯晚疫病水平抗性紧密相关的候选基因,以期作为分子标记辅助选择的重要标记。【方法】以BCT和PCC1两个马铃薯群体为材料,对38个晚疫病菌诱导表达的ESTs和基因进行定位,再将定位结果与已定位的QTL位点进行比对。【结果】11个候选基因的引物在两个群体中扩增出13个多态性位点,其中12个多态性位点定位到遗传连锁图谱上。定位结果与QTL进行比较显示,07-F08-P1-564位于晚疫病QTL区域。【结论】07-F08-P1-564与马铃薯晚疫病水平抗性紧密相关。定位的候选基因丰富了马铃薯的连锁群,可作为遗传连锁图谱构建的桥梁,同时也为筛选重要抗性候选基因奠定了基础。

[Objective]The present studies were carried out to explore putative candidate genes associated with horizontal resistance to late blight disease of potato in order to utilize them in marker-assisted selection. [Method ]Thirty eight ESTs and genes, showing induced expression by Phytophthora infestans infection, were selected for mapping in two potato populations BCT and PCC1. The location results were used to compare with the known QTL loci. [Result]In total, 11 candidate genes amplified 13 polymorphic bands in both the populations, of which 12 bands were mapped on the chromosome. The comparison of mapping results with the known QTLs indicated that 072F08-P1-564 was located inQTL area of late blight resistance. [Conclusion]It was concluded that 07-F08-P1-564 was related to horizontal resistance of potato late blight. These mapped candidate genes enriched linkage maps of potato arid may be used as a bridge for genetic mapping of potato populations.