脉冲场凝胶电泳在沙门菌鉴定中的应用

Application of pulsed-field gel electrophoresis in the identification of salmonella

目的探讨脉冲场凝胶电泳(PFGE)在沙门菌分型鉴定中的应用价值。方法用PFGE方法对一起食物中毒分离出的68株肠炎沙门菌菌株进行分子分型。结果 180份标本经传统生化和血清分型鉴定出的68株肠炎沙门菌,通过PFGE分型后,均产生14条电泳条带,且分子量大小一致。通过BioNumerics软件的数据化处理分析,得到聚类关系树,68株肠炎沙门菌指纹图谱的相似性为100%,与H9812的相似性为65.65%。结论 PFGE是沙门菌分型鉴定的有效手段,可用于细菌性食物中毒传染源的追踪。

Objective To investigate the value of pulsed-field gel electrophoresis（PFGE） in the typing identification of salmonella.Methods PFGE method was used to 68 salmonella strains molecular typing identification isolated from food poisoning.Results 68 strains of salmonella enteritidis identified by traditional biochemical and the serotyping from 180 specimens,after typing by PFGE,14 electrophoretic bands were produced,and had the same molecular weight.The data was analyzed by BioNumerics software,clustering trees were obtained.The similarity of fingerprints was 100% in 68 salmonella enteritidis,65.65% similarity with the H9812.Conclusion PFGE is an effective means in the typing identification of Salmonella,and can be used to trace the source of infection in bacterial food poisoning.