云南省东北等地区蚊虫及蚊媒病毒调查研究

Investigation of mosquitoes and mosquito-borne viruses in northeast Yunnan province

目的了解滇东北等地区蚊虫及蚊传虫媒病毒分布特点,为虫媒病毒病防治提供科学依据。方法 2009年在滇东北等地区的6个县采集蚊虫标本;蚊虫经分类鉴定后,用细胞培养法分离病毒,对病毒分离物进行分子生物学鉴定。结果共采集到4属(库蚊、按蚊、阿蚊、伊蚊)24种18562只蚊虫,其中三带喙库蚊、中华按蚊为主要蚊种,构成比分别为58.37%和28.45%;从蚊虫标本中分离到15株病毒,经分子生物学鉴定,其中2株(YN0911和YN0967)为基因Ⅰ型流行性乙型脑炎(乙脑)病毒,均分离自三带喙库蚊;1株(YN0922)为版纳病毒,分离自中华按蚊;12株为淡色库蚊浓核病毒,其中9株分离自三带喙库蚊,3株分离自中华按蚊。结论三带喙库蚊和中华按蚊为调查地区的优势蚊种,并携带乙脑病毒、版纳病毒和淡色库蚊浓核病毒;滇东北地区首次分离到乙脑病毒。

Objective To investigate the distribution patterns of mosquitoes and mosquito- borne viruses in Yunnan province to provide evidence for prevention and control of mosquito-borne diseases. Methods In 2009, mosquito samples were collected from 6 counties of Yunnan province. Mosquitoes were first classified, followed by cell culturing for viral isolation for molecular identification. Positive isolates were identified using SDS- PAGE and RT-PCR. The genomes of isolates were also, sequenced for phylogenetic analysis. Results A total of 18 562 mosquitos of 4 genera （Culex, Anopheles, Armigeres, Aedes） and 24 species were collected. Cx. tritaeniorhynchus and An. sinensis were the major species, comprising 58.37% and 28.45% of the total population, respectively. Fifteen strains of viruses were isolated from the mosquito pools. RT-PCR and phylogenetic analysis revealed two viral strains from Cx. tritaeniorhynchus, identified as Japanese encephalitis virus （JEV, Genotype I ）. One strain isolated from An. sinensis was identified as Banna virus. Twelve vrial strains were identified as Cx. pipiens pallens densovirus （CppDNV）, of these, nine strains were isolated from Cx. tritaeniorhynchus, and three strains from An. sinensis. Conclusion Cx. tritaeniorhynchus and An. sinensis were the predominant species in the investigated areas. JEV, Banna virus and CppDNV were isolated. In northeast Yunnan, JEV was isolated for the first time.