一对可以用于喜马拉雅旱獭性别鉴定的引物筛选

Screening of primers for sex identification of Marmota himalayana

目的从用于黄鼠性别鉴定的sry和smcY引物中筛选出能够用于喜马拉雅旱獭性别鉴定的引物。方法用1对sry引物和4条smcY引物的3对组合对已知性别的雌雄各10只喜马拉雅旱獭基因组DNA进行扩增,同时,用1对微卫星引物来验证反应体系的有效性。结果在验证了PCR反应有效性的前提下,sry引物从100～1000bp间扩增出多个条带,且未发现雄性特异性条带,smcY引物组合1在雄性旱獭中扩增出100bp左右的亮带,但是部分雌性样品中在600bp左右扩增出较弱的条带,组合2未扩增出任何条带,组合3的所有雄性在250bp处扩增出明亮的条带,雌性个体中未见任何条带。结论 smcY引物组合3可以用于喜马拉雅旱獭性别鉴定。

Objective To select a primer set tor sex identification of Marmota himatayana trom the sry and smcY primers used in ground squirrels. Methods One pair of sry primers and four smcY primers （used in 3 different combinations） were used to amplify the genomes of 10 M. himalayana of known sexes. One pair of microsatellite primers were used to verify the method. Results The sry primers amplified several bands from 100 bp to 1000 bp, but no male-specific bands were found. Combination 1 of smcY primers amplified a bright band of about 100 bp only in males, but a weak band of about 600 bp was also amplified in some females. Using smcY primer combination 2 yielded no amplified bands. Combination 3 amplified a bright band of about 250 bp in all males, but no band was observed in females. Conclusion The gender selectivity of smcY primer combination 3 means it could be used for sex identification of M. himalayana.