采自家蝇成虫的尖突巴科霉形态观察

Morphological description of Batkoa apiculata collected from adult houseflies

目的描述采自家蝇成虫的尖突巴科霉形态特征,为控制家蝇提供科学依据。方法醋酸地衣红染色法观察尖突巴科霉的形态,分别显微测量分生孢子、分生孢子梗和假根直径,并摄影。结果室内保湿培养12h后,从家蝇成虫尸体的头部、胸部和腹部节间膜长出分生孢子梗,产生分生孢子。尖突巴科霉初生分生孢子球形,单囊壁,多核,>13个,(36.2±2.0)μm×(29.3±2.5)μm〔(31.9～45.6)μm×(23.9～40.3)μm〕,L/D为1.2±0.1(1.1～1.5);次生分生孢子形似初生分生孢子,但略小(27.8±1.9)μm×(24.1±1.5)μm〔(23.9～31.8)μm×(19.3～26.2)μm〕,L/D为1.2±0.1(1.0～1.4)。分生孢子梗棒状,不分枝,Φ(14.2±1.5)μm(11.8～18.6μm)。假根单菌丝状,端部分化为盘状固着器。假囊状体和休眠孢子未见。结论国内未见尖突巴科霉感染家蝇的报道,该菌对家蝇可能具有控制潜力。

Objective To describe the fungal species Batkoa apiculata found in adult Musca domestica. Methods After aceto-orcein staining, the diameters of conidia, conidiophores and rhizoids of B. apiculata were measured and photographed. Results Cultured at high humidity for 12 hours, conidiophores and conidia of the fungus emerged from the head, thorax and intersegmental membranes of the abdomen of the fly specimen. Primary conidia were spherical with a papilla basad, unitunicate, multinucleate （more than 13）, （36.2±2.0）μm×（29.3±2.5） μm [（31.9-45.6） μm × （23.9-40.3） μm], L/D 1.2±0.1 （1.1-1.5）. Secondary conidia were small, similar to primary conidia, （27.8±1.9）μm×（24.1±1.5）μm （（23.9-31.8）μm×（19.3-26.2）1xm], L/D 1.2±0.1 （1.0-1.4）. Conidiophores were club-shaped and unbranched, （14.2±1.5） μm （11.8-18.6 μm）. Rhizoids were monohyphal with a disc- like structure at apex. Pseudocytidia and resting spores were not found. Conclusion B. apiculata collected from M. domestica is new to China. The fungus may serve as a potential agent for the control M. domestica.