秦川牛微卫星基因位点遗传多样性分析

The Analysis of Genetic Diversity of Microsatellite Loci in Qinchuan Cattle

利用PCR技术和电泳银染技术检测了秦川牛BM2113、IDVGA2、TGLA44、IDVGA27、ETH10、ILSTS005、和IDVGA46等7个微卫星位点的多态性分布,计算了各个基因座的表观及期望杂合度、有效等位基因数、多态信息含量、固定指数和Shannon信息熵。结果显示,秦秦川牛群体的遗传杂合度较高,遗传多样性丰富,所选微卫星位点可用于秦川牛遗传多样性评估。

The polymorphism distributions of microsatellite loci,BM2113,IDVGA2,TGLA44,IDVGA27,Beef Cattle Improvement Centre in China,Yangling Shaanxi 712100） ETH10,ILSTS005 and IDVGA46 were detected in Qinchuan cattle by Polymerase Chain Reaction and multiplex gel electrophoresis followed by silver staining.The results indicated that genetic heterozygosity of Qinchuan cattle was high,genetic diversity Was rich.The selected microsatellite loci can be used to evaluate genetic diversity of Qinchuan cattle.