儿童哮喘急性发作期外周血单核细胞NF-κB活性与血清IL-4、IgE水平变化及临床意义

Changes and Clinical Significances between NF-κB Activity in Peripheral Blood Mononuclear Cells and Serum Levels of Interleukin-4 and Immunoglobulin E in Asthmatic Children

目的:探讨儿童哮喘急性发作期外周血单核细胞NF-κB活性与血清IL-4、IgE的水平变化及临床意义。方法:本研究设正常对照组52例,哮喘急性发作组84例,应用ELISA法检测血清IL-4、IgE水平,并提取外周血单核细胞用电泳迁移率分析法(EMSA)测定NF-κB活性。结果:在儿童哮喘急性发作组,外周血单核细胞NF-κB活性(0.793±0.062)明显高于正常对照组(0.643±0.071)(P<0.01);儿童哮喘急性发作组血清IL-4(108.2±41.7)ng/L显著高于正常对照组(38.2±15.3)ng/L;儿童哮喘急性发作组血清IgE(165.3±50.7)KU/L也显著高于正常对照组(45.8±14.2)KU/L;儿童哮喘急性发作组NF-κB活性与血清IL-4、IgE水平呈正相关(r=0.624、0.791,P<0.01)。结论:儿童哮喘急性发作期外周血单核细胞NF-κB活性明显增加,可能参与了放大炎症信号及调节炎症持续过程,抑制NF-κB活性可能下调IL-4、IgE的表达,能在较高的水平上调控产生炎性因子的总体环节,更有效地控制哮喘。

Objective： To explore the changes of NF-κB activity in peripheral blood mononuclear cells（PBMCs） and serum levels of IL-4 and IgE in asthmatic children and their clinical significances.Methods：The NF-κB activity was measured by electrophoretic mobility shift assay（EMSA） in 84 patients with exacerbating asthma（asthmatic children group） and 52 normal subjects（control group）.Results： The NF-κB activity in asthmatic children group（0.793±0.062） was significantly higher than the control group（0.643±0.071）（P0.01）.The serum levels of IL-4（108.2±41.7） ng/L in asthmatic children group was predominantly higher than the（38.2±15.3）ng/L in control group（P0.01）.The serum levels of IgE（165.3±50.7）KU/L in asthmatic children group was also higher than the（45.8±14.2） KU/L in control group（P0.01）.The NF-κB activity had positive correlations with the serum levels of IL-4 and IgE（r=0.624,0.791,P0.01）respectively.Conclusions： These results indicate that the NF-κB activity in asthmatic children is increased,then amplifies the inflammatory signals and regulates the process of inflammation.The general steps of the production of inflammation factors can be controlled at a much higher level by inhibiting the NF-κB activity,and then down regulating the expressions of IL-4 and IgE,so childhood asthma can be treated more effectively by this mechanism.