LAS高效降解菌的分离鉴定及其降解性能的初步研究

Preliminary Study on Isolation of High Effective LAS Degrading Strain and Its Degradability

从环境中筛选分离出能高效降解直链烷基苯磺酸钠（LAS）的菌株,研究其降解性能,以期为LAS污染治理提供菌种资源。利用富集培养技术从重庆市某地区经含洗涤剂污水长期浸泡的污泥中分离获得1株对直链烷基苯磺酸钠（LAS）具有较强降解能力的菌株,命名为L1。通过对菌株形态、生理和生化特性分析,初步鉴定菌株L1为黄单胞菌属（Xanthomona sp.）。经研究,该菌的生长和LAS降解率在pH5.0~7.5之间比较稳定,其中在pH为7.0时效果最佳,并且具有一定的酸碱调节能力。LAS浓度为150mg/L,是该菌生长和LAS降解的最适浓度,48h菌体生长量测定的吸光值可达0.413,LAS降解率可达87.64%,表明菌株L1具有治理LAS污染的潜在应用价值。

In order to supply microorganism resources to LAS pollution control, the author dealt with isolating a strain with high degradability to degrade LAS from environment and studying on its degradability. The strain with high degradability to degrade LAS, isolated through enrichment culture method from sludge soaked in detergent sewage for a long time in a region of Chongqing was called L 1 . The strain L 1 was preliminarily recognized as Xanthomonas sp. by the analysis of its morphology, physiology and biochemical characteristics. After studying, its growth and degradation rate of LAS was comparatively stable when the pH ranged between 5.0-7.5 and it could reach its summit when the pH was 7.0. What＇s more, it had certain ability on pH regulations. When the consistency of LAS was 150 mg/L, it was the most favorable consistency to the growth of L 1 and the degrading of LAS, the growth of L 1 during 48 h could reach 0.413 （OD 460 ） and the degrading rate was as high as 87.64%. All this showed that the strain L1 had certain potential in LAS pollution control.