双氢青蒿素人对急性髓系白血病HL-60细胞凋亡的诱导作用

The induction dihydroartemisinin on apoptosis of human acute myeloblast leukemia HL-60 cells in vitro

目的探讨双氢青蒿素诱导人急性髓系白血病HL-60细胞凋亡的作用和机制。方法用二甲氧唑黄细胞增殖及细胞毒性检测试剂盒检测双氢青蒿素对HL-60细胞生长的抑制作用,Annexin V-FITC/PI染色流式细胞仪检测细胞凋亡,流式细胞仪检测细胞内线粒体膜电位变化,分光光度法检测细胞Caspase-3活性变化。结果双氢青蒿素对HL-60细胞生长有抑制作用,呈剂量依赖性,并能诱导HL-60细胞凋亡,使线粒体膜去极化,增强Caspase-3活性。结论双氢青蒿素能抑制人急性髓系白血病HL-60细胞生长,并诱导细胞凋亡,可能与线粒体凋亡通路有关。

Objective To investigate the role of dihydroartemisinin in the apoptosis of human acute myeloblastic leukemia HL - 60 cells. Methods The growth inhibition of HL - 60 cells treated with dihydroartemisinin was assessed by XTT cell proliferation and cytotoxicity assay kit. The apoptosis and mitochondria membrane potential of HL - 60 cells were assessed by flow cytometry with AnnexinV - FITC/PI and JC - 1 staining, respectively. The activity of Caspase - 3 was measured by ELISA. Results Dihydroartemisinin inhibited HL - 60 cells proliferation in a concentration - dependent manner, and induced the apoptosis of HL- 60 cells via depolarizing mitochondria membrane and activating Caspase- 3. Conclusion Dihydroartemisinin inhibits HL- 60 ceils proliferation and induces HL -60 cells apoptosis, which may be correlated with mitochondria apoptosis pathway.