人工心脏瓣膜材料细菌粘附的体外实验

Study of Bacterial Adhesion to Prosthetic Valve Materials in Vitro

通过体外实验，评价人工心瓣心内膜炎常见致病菌对人工心瓣材料粘附情况，初步建立人工心瓣材料细菌粘附的测定方法。采用平板菌落计数法、１２５Ｉ标记细菌放射性测定法，测定金黄色葡萄球菌、表皮葡萄球菌、大肠杆菌、绿脓杆菌对涤纶、热解碳、聚四氟乙烯的粘附情况。结果四种细菌对三种材料粘附能力与细菌生长曲线基本一致。四种细菌对涤纶粘附较强，特别是金黄色葡萄球菌对涤纶粘附不随生长曲线进入衰亡期而减少。表皮葡萄球菌对热解碳的粘附最强，绿脓杆菌对热解碳的粘附维持在高水平状态。大肠杆菌、绿脓杆菌对聚四氟乙烯粘附最强。结论：不同细菌对同一种人工心瓣材料或同一种细菌对不同的人工心瓣材料有不同粘附能力。

This article reports a study of the bacterial adhesion to prosthetic valve materials in vitro. The method for assessing the degree of bacterial adhesion to prosthetic valve materials was established primarily.The capacities of staphylococcus aureus(SA), staphylococcus epidermidis(SE), Escherichia Coli(EC) and Pseudomonas aeruginosa(PA) for adhesion to Dacron, Pyrolytic carbon and polytetrofluoroethylene(PTFE) were quantitatively determined by the plate counting and Gamma ray counting of 125 I radiolabeled bacteria in vitro. The results showed that the capacities of four types of bacteria for adhesion to Dacron, Pyrolytic carbon and PTFE coincided with the bacterial growth curve. The capacities of four bacteria for adhering to Dacron were stronger. The adhesion of SE to Pyrolytic carbon was the strongest. The adhesion of PA Kept up a high level.The capacities of EC and PA for adhering to PTFE were the strongest. The results indicate that the capacities of different types of bacteria for adhesion to the same prosthetic valve material are different, and the capacities of one type of bacteria for adhesion to different materials are also different.