非小细胞肺癌p53基因突变检测及临床病理分析

DETECTION OF p53 GENE MUTATION IN PRIMARY NON-SMALL CELL LUNG CANCER AND THE CLINOPATHOLOGICAL ANALYSIS

目的 探讨ｐ５３基因点突变在非小细胞肺癌中的发生情况及临床病理联系。方法 运用聚合酶链反应（ＰＣＲ）－单链构象多态性分析方法（ＳＳＣＰ）检测原发性非小细胞肺癌的ｐ５３ 基因第５－８外显子点突变。结果 ８例良性肿瘤均无点突变发生，４０ 例肺癌中１９例（４７．５％ ）有点突变发生，点突变发生与临床分期和淋巴结累及程度明显相关（Ｐ＜ ０．０２）。结论 ｐ５３基因点突变在非小细胞肺癌的发生和进展中起着相当重要的作用。

Objective To investigate the significance of p53 gene mutations in non small cell lung cancer with focusing on the four highly conserved regions of the p53 gene.Methods Point mutations of the p53 geng were evaluated by polymerase chain reaction single strand conformation polymorphism(PCR SSCP)analysis in 40 lung cancers and 8 benign lung diseases.Results Mutations of the p53 gene were founded in 19 of 40 lung cancer samples (47.5%).The frequency of the p53 gene mutations varied among different clinical stages(P<0.005),with or without nodal involvement (P<0.025)in non small cell lung cancers.Conclusion The results showed that p53 gene mutation may be associated with the carcinogenesis and progression of non small cell lung cancers.