鼻咽癌患者P^(16)/MTS_1抑癌基因失活研究

Studies of inactivation of P^(16)/MTS_1 gene in nasopharyngeal cancer patients

目的：以原发性鼻咽癌（NPC）患者为研究对象，检测鼻咽癌组织中P16／MTSI抑癌基因失活情况；研究鼻咽癌发生发展规律。方法：采用PCR、多重PCR、限制性内切酶解一PCR方法对34例鼻咽癌患者和34例非肿瘤（腺样体增殖及慢性炎症）患者，进行P基因纯会缺失和异常甲基化检测。结果：34例NPC患者纯缺失率为8．82％（3／34），异常甲基化率为38．24％（13／34），34例非肿瘤患者纯缺失未检出（0／34），异常甲基化率为8．82％（3／34）。结论：本研究鼻咽癌患者P16／MTSI抑癌基因总失活率为47．06％（16／34），表明P16基因失活在鼻咽癌患者中是较为常见的基因变化。鼻咽癌患者中甲基化异常是P16基因失活的另一重要途径，值得引起重视。

To investigate the incidence of P16/MTS1 (multiple tumor sup-pressor l )gene inactivation in the tissues from nasopharyngeal cancer (NPC)patients.Methods. Thirty-four patients with NPC and 34 patients with non-malignancies (ade-noidism or chronic infection)were studied to detect P16 gene homozygous deletion and ab-normal methylation by polymerase chain reaction(PCR),multiplex PCR and restrictionenzyme PCR. Results. P16 gene homozygous deletion was detected in 3 of 34 NPC patients(8.82%), abnormal methylation was detected in 13 of 34 NPC patients (38. 24%) ;whereas in the 34 patients with non-malignancies,P16 gene homozygous deletion was notdetected,abnormal methylation was detected in 3 of 34 patients (8. 82%). Conclusion.The overall incidence of P16/MTS, gene inactivati0n in NPC patients was 47. 06% (16/34),it indicated that P16 gene inactivation was the common incidence in NPC patients,andit should be noted that P16 gene abnormal methylation was another important pathway ofP16 gene inactivation.