喉癌引流淋巴结中巨噬细胞对自体肿瘤杀伤活性检测

Determination of the tumoricidal activity of macrophages to autologous laryngeal carcinoma

本实验采作细胞培养技术，从喉癌患者颈廓清淋巴结中提取的巨噬细胞（Mphi）作效应细胞，喉癌患者自体癌细胞及K562细胞作靶细胞，分别混合培养（两组均加PHA作为刺激原），结果表明：喉癌患者颈部肿大淋巴结Mphi对自体癌细胞（35例）及K562细胞（30例）均有杀伤能力；此外，我们还对比了未转移淋巴结（N0组，18例）与转移淋巴结（N+组，17例）MPhi杀伤能力，结果N0组高于N+组，差异非常显著（P＜0．01）。结果提示，喉癌患者颈部引流淋巴结中尚存在着免疫活性细胞，并具有肮肿瘤免疫潜能，该实验为临床合理实施颈廓清术及将活化的Mphi应用于肿瘤免疫治疗提供理论依据。

Autologous laryngocarcinoma cells and K562 cells were used as targetcells to evaluate the tumoricidal activity of macrophages which were isolated from re-gional lymph nodes of laryngocarcinorna patients by the cell culture technique(at an ef-fector to target cell ratio of lO: 1). Both of the groups were added PHA as stimulator.The results indicated that macrophages had certain tumoricidal activity to both autolo-gous tumor cells (35 cases ) and K562 cells (3O cases ). The tumoricidal activities ofmacrophages were observed comparatively between non-metastatic lymph nodes (N0group )and metastatic lymph nodes (N + group). The results demonstrated that the tumo-ricidal activity of N0 group was significantly higher than that of N+ group. The resultsshowed that there were immune-active cells in the lymph nodes of the patients withlaryngocarcinoma and provided theoretical basis of using rnacrophages in anti-tumor im-munologic treatment,and they also suggested that neck desection should be done ratio-nally to the laryngocarcinoma patients.