良恶性肿瘤P^(53)蛋白检测在快速诊断中的应用

The Application of P^(53) Protein Detection in The Rapid Diagnosis of Malignant and Benign Tumors

目的：探讨常规石蜡切片的前期处理对P^53基因产物免疫组化结果的影响以及P^53蛋白在冰冻切片的快速检测在良恶性肿瘤鉴别诊断中的应用价值。方法：选用49树良性病变组织和引例恶性病变组织作PS^53蛋白的检测，冰冻切片采用Envision法结合微波，常规石蜡切片选用S－P法。结果：两种方法表达率几乎一致，且恶性病变组织P^53蛋白的阳性率明显高于良性病变组织。结论：在冰冻切片中P^53基因表达的快速检测，对良恶性病变的鉴别诊断具有指导意义。

objective: To approach the effect of pretreatment of routine paraffin wax section on the immunohistochemical results of p' gene production and thc application value of p' protein in differcntial diagnooisbetween malignant and benign tumors. Methods: Ps' proteins in 49 cases of bcnign lesion and 5l cases of malignant 1csion were detected by using immunohistochemistry S - P method in paraffin wax section and by using Envision technique combined with microwave in frozcn scction. Results: There is no significant differcncc betweentwo mctheds in the expressing rate of p', but the positive rate of p' in malignant lesion is significantly highcrthan that in benign Icsion. Conclusion: The fast detection of p' gene cxpression in frozen section is valuablc inthe diffcrcntial diagnosis betwccn maIignant and benign lcsion.