大肠杆菌代谢物组成的核磁共振分析

Nuclear Magnetic Resonance for Analysis of Metabolite Composition of Escherichia coli

为了认识重要模式生物大肠杆菌的内源性代谢物组成,本研究综合使用一维和二维高分辨核磁共振波谱技术,系统测定了大肠杆菌的代谢物种类和含量。结果表明:核磁共振方法可检测到分别来自多个代谢途径的氨基酸、有机羧酸、糖类、核苷及其衍生物等40多种高丰度代谢物。葡萄糖是稳定期大肠杆菌中含量最丰富的物质;谷氨酸、甜菜碱、腐胺和核糖-5-磷酸的含量次之;其它代谢物的含量都相对较低。这些结果证实NMR技术能同时有效检测细菌中含量丰富且极性各异的初级和次级代谢产物。

To understand the composition of endogenous metabolites of Escherichia coli,an important model organism,a series of high-resolution NMR spectroscopic methods were employed to analyze the metabolites of this strain qualitatively and quantitatively.We found that E.coli metabolome was dominated by more than 40 abundant metabolites including amino acids,organic acids,sugars,nucleoside and its derivatives involving many different metabolic pathways.We further found that glucose level was the highest in stationary-phase E.coli while glutamate,betaine,putrescine and ribose-5-phosphate levels were higher than other metabolites.Our results indicated that NMR method was effective to simultaneously detect the abundant bacterial metabolites of different polarity including both the primary and secondary metabolites.