应用粪便COX-2mRNA检测结直肠癌的研究

Fecal COX-2 mRNA as a Biomarker for Detecting Colorectal Cancer

目的探讨应用粪便COX-2 mRNA为标志物检测结直肠癌的临床意义。方法连续收集43例结直肠癌病人和39例健康正常人的粪便标本各1份,采用巢式逆转录聚合酶链反应检测粪便中脱落细胞COX-2 mRNA的表达,分析应用粪便COX-2 mRNA检测结直肠癌的效率。结果 43例结直肠癌粪便中有27例COX-2 mRNA表达呈阳性,阳性率62.8%;39例正常对照组中有6例COX-2 mRNA表达呈阳性,阳性率15.4%,检出率差异有统计学意义(χ2=19.112,P<0.01)。粪便COX-2 mRNA检测结直肠癌的受试者工作特征曲线下面积为0.737,95%置信区间为(0.627,0.847)。结论 COX-2 mRNA可作为一种肿瘤标志物在粪便中检出,其对检测结直肠癌具有较好的诊断价值。

Objective To investigate the clinical significance of fecal COX-2 mRNA as a biomarker for detectiing colorectal cancer.Methods Fecal samples of 43 colorectal cancer patients and 39 heathy people were consecutively collected.Net reverse-transcription polymerase chain reaction was used to detect the expression of COX-2 mRNA in the fecal exfoliated cells.Efficiency of fecal COX-2 mRNA for detecting colorectal cancer was analyzed.Results COX-2 mRNA was detected in 27 out of 43 colorectal cancer fecal samples,with the positive rate of 62.8%.And it was 6 out of 43 in the normal control（15.4%）.There was significant difference between the two groups（χ2 = 19.112,P 0.01）.The area under the curve of ROC was 0.737,with the 95% confidence section between 0.627 and 0.847.Conclusions COX-2 mRNA is a tumor marker which can be detected in the feces,and its diagnosis value is medium for colorectal cancer.