p38和uPA在上皮性卵巢癌组织中的表达及意义

Expression of Mitogen Activated Protein Kinase p38 and Urokinase-type Plasminogen Activator in Ovarian Cancer

目的探讨丝裂原活化蛋白激酶(p38)与尿激酶型纤溶酶原激活剂(uPA)在卵巢肿瘤组织中的表达及相关性。方法选取上皮性卵巢癌组织60例,良性上皮性卵巢肿瘤组织30例,正常卵巢组织30例。采用免疫组织化学方法检测p38与uPA在上述不同组织中的表达。结果 p38和uPA在上皮性卵巢癌组织、良性上皮性卵巢肿瘤组织及正常卵巢组织中的表达率分别为75.0%,36.7%,26.7%和68.3%,40%,30%。卵巢癌组与良性卵巢肿瘤组及正常卵巢组比较差别均有显著性(P<0.05),而良性卵巢肿瘤组织与正常卵巢组比较差别无显著性(P>0.05);二者的表达与上皮性卵巢癌组织临床分期、组织学分级、及淋巴结转移显著相关(P<0.05),且随临床分期增加、分化降低及淋巴转移而增强(P<0.05)。p38和uPA在卵巢上皮性肿瘤中的表达密切相关(r=0.827,P<0.05)。结论 p38和uPA与卵巢癌发生发展生物学行为有关,二者联合检测有助于卵巢癌早期诊断和预后判断。

Objective To investigate the expression and correlation of p38 and uPA in ovarian cancer tissue.Methods 60 cases of epithele ovarian cancer,30 cases of ovarian benign neoplasm of ovary and 30 cases of normal ovarian tissue were chosen.Methods of immunohistochemistry were adopted to detect the different expressions of p38 and uPA in the above tissue.Results The positive staining rate of p38 and uPA in ovarian carcinoma,benign neoplasm of ovary and normal tissue were 75 %,36.7%,26.7% and 68.3%,40%,30%.Groups of ovarian cancer compared with the group of ovarian benign neoplasm and that of normal ovarian tissue,the distinction was significant（P 0.05）.The distinction between ovarian benign neoplasm and normal ovarian tissue is not significant（P 0.05）.Both expressions had positive correlation with ovarian cancer tissue clinical stage,histological grade and lymph metastasis（P 0.05）,and the correlation was enhanced with clinical stages,differentiation reduction and lymph metastasis（P 0.05）.The expression of p38 and uPA was among the significant positive correlation（r=0.827,P 0.05）.Conclusion p38 and uPA might be associated with the biological behaviors of ovarian cancer,and the united detection of the two contributes to the early diagnosis of ovarian cancer prognosis estimate.