幽门螺杆菌清除前后胃粘膜C-myc蛋白和表皮生长因子受体的变化

Expression of C-myc gene protein and epidermal growth factor receptor in gastric mucosa pre and post Helicobacter pylori clearance

目的 了解幽门螺杆菌（ Ｈｐ） 感染对胃粘膜Ｃｍｙｃ 基因蛋白和表皮生长因子受体（ＥＧＦＲ） 表达的影响．方法 应用流式细胞术和免疫荧光染色技术，对Ｈｐ 清除前后胃粘膜Ｃｍｙｃ 基因蛋白和ＥＧＦＲ 进行定量测定，用免疫组化法研究增殖细胞核抗原（ＰＣＮＡ） 在组织中的表达．结果 Ｈｐ 阳性患者胃粘膜细胞Ｃｍｙｃ 蛋白和ＥＧＦＲ 的阳性细胞百分率和平均荧光强度显著高于 Ｈｐ 阴性患者，经治疗Ｈｐ转阴者上述指标显著下降，而Ｈｐ 仍为阳性者上述指标则无明显变化． 胃粘膜ＰＣＮＡ 标记指数与Ｃｍｙｃ 蛋白和ＥＧＦＲ 呈一致性改变．结论 Ｈｐ 感染导致的Ｃｍｙｃ 蛋白和ＥＧＦＲ 的过度表达，可能对细胞增殖和癌变具有促进作用．

AIM To investigate the effect of Helicobacter pylori infection on the expression of C myc gene protein and epidermal growth factor receptor (EGFR) in gastric mucosa. METHODS The expression of C myc gene protein and EGFR was quantitatively studied by flow cytometry and indirect immunofluorescence methods, proliferative cell nuclear antigen (PCNA) was analysed by immunochemistry method. RESULTS The percentage of positive cells and mean fluorescence intensity of C myc protein and EGFR were significantly increased in Hp infected mucosa and significantly decreased after Hp clearance with triple therapy. This change was similar to that in proliferative activity indicated by PCNA labeling index. CONCLUSION The over expression of C myc gene protein and EGFR may be the molecular basis for hyperproliferation of gastric mucosal epithelium induced by Hp infection.