摘要
目的 了解幽门螺杆菌( Hp) 感染对胃粘膜Cmyc 基因蛋白和表皮生长因子受体(EGFR) 表达的影响.方法 应用流式细胞术和免疫荧光染色技术,对Hp 清除前后胃粘膜Cmyc 基因蛋白和EGFR 进行定量测定,用免疫组化法研究增殖细胞核抗原(PCNA) 在组织中的表达.结果 Hp 阳性患者胃粘膜细胞Cmyc 蛋白和EGFR 的阳性细胞百分率和平均荧光强度显著高于 Hp 阴性患者,经治疗Hp转阴者上述指标显著下降,而Hp 仍为阳性者上述指标则无明显变化. 胃粘膜PCNA 标记指数与Cmyc 蛋白和EGFR 呈一致性改变.结论 Hp 感染导致的Cmyc 蛋白和EGFR 的过度表达,可能对细胞增殖和癌变具有促进作用.
AIM To investigate the effect of Helicobacter pylori infection on the expression of C myc gene protein and epidermal growth factor receptor (EGFR) in gastric mucosa. METHODS The expression of C myc gene protein and EGFR was quantitatively studied by flow cytometry and indirect immunofluorescence methods, proliferative cell nuclear antigen (PCNA) was analysed by immunochemistry method. RESULTS The percentage of positive cells and mean fluorescence intensity of C myc protein and EGFR were significantly increased in Hp infected mucosa and significantly decreased after Hp clearance with triple therapy. This change was similar to that in proliferative activity indicated by PCNA labeling index. CONCLUSION The over expression of C myc gene protein and EGFR may be the molecular basis for hyperproliferation of gastric mucosal epithelium induced by Hp infection.
出处
《世界华人消化杂志》
CAS
1999年第12期1018-1019,共2页
World Chinese Journal of Digestology
关键词
幽门螺杆菌
胃粘膜
C-MYC基因
表皮生长因子
Helicobacter pylori
gastric mucosa
C myc gene protein
receptor
epidermal growth factor