血清纤维化指标与肝组织病理和肝组织纤维定量关系的实验研究

Experimental study on serum fibrosis markers and liver tissue pathology and hepatic fibrosis in immuno damaged rats

目的 本研究观察实验大鼠血清纤维化三项指标与肝组织病理和纤维定量之间的确切关系，探讨其临床应用价值．方法 利用人血清蛋白免疫损伤Ｗｉｓｔａｒ 大鼠造成肝纤维化模型，综合分析模型组、促肝细胞生长因子治疗组和正常对照组之间血清肝纤维化指标、肝组织病理改变、肝组织胶原纤维和网状纤维病理图象定量分析等变化．结果 模型组血清肝纤维化指标（ＰＣⅢ，ＨＡ，ＬＮ） 均高于对照组，差异有显著意义（ Ｐ＜ ０－０５ 或Ｐ＜ ０－０１） ． 光镜下肝组织病理变化，肝组织胶原纤维和网状纤维病理图象定量分析模型组结果均高于对照组（ Ｐ＜ ０－０５） ．结论 血清三项指标与肝组织病理和纤维定量呈现一致性改变，显示良好的正相关．

AIM In order to determine the clinical value, we observed the relation among the serum fibrosis markers, hepatic tissue pathology and hepatic fibrosis in quantity. METHODS Liver fibrosis model group were Wistar rats immuno damaged by human serum albumin, level of serum precollagen Ⅲ (PCⅢ), hyaluronic acid (HA), LN, change of hepatic tissue pathology and analysis of collagenous and reticular fibers pathology picture in the liver tissue were comprehensively evaluated among model group and treatment group as well as control group. RESULTS Serum PCⅢ, HA, and LN of the model group were obviously higher than control group, the difference was significant ( P <0 05 or <0 01 ). Pathology change and quantity of collagenic fibers and reticular fibers in liver tissue were higher than control group ( P <0 01 or <0 05 ). CONCLUSION The results demonstrated that changes in serum fibrosis markers and hepatic tissue pathology as well as collagenous fibers and reticular fibers were consistent with each other.