摘要
以羟基取代卟啉为原料,通过控制反应过程中双卟啉磷酰氯的合成条件,采用一锅法合成了5个异环磷酰胺氮芥卟啉二聚体.所有化合物均经过MS、1H NMR、31P NMR和元素分析确定结构.细胞吸附实验和细胞毒性实验结果表明,异环磷酰胺氮芥卟啉二聚体可以选择性富集和杀死肿瘤细胞.利用荧光光谱研究了异环磷酰胺氮芥卟啉二聚体与牛血清白蛋白(BSA)的相互作用,结果表明卟啉二聚体对BSA的荧光有较强的静态猝灭作用,可以生成稳定的卟啉二聚体-BSA复合物.
Ifosfamide is one of the most important clinical alkylating agent due to its frequent use in cancer chemotherapy.It does not exhibit any cytotoxic activities in vitro,and requires hepatic oxidative metabolism in vivo to generate an alkylating ifosfamide mustard,which is regarded as the ultimate intracellular alkylating metabolite.However,its clinical uses are restricted due to they can not distinguish tumor cells from normal cells.Porphyrins could selectively accumulate in tumor tissues than in normal tissues.Based on these facts,an efficient one-pot method for the synthesis of ifosfamide mustards porphyrin dimers was achieved by treating hydroxyl substituted porphyrin first with POCl3 then with primary amine.The selective synthesis of ifosfamide mustards porphyrin dimers were achieved simply by controlling the ratio of POCl3 vs.hydroxyl porphyrin and the reaction condition during the phosphonation step.Five ifosfamide mustards porphyrin dimers were synthesized accordingly by this one-pot procedure and their structures were affirmed by MS,1H NMR,31P NMR and elemental analysis.The MTT tests and the uptake tests showed that ifosfamide mustards porphyrin dimers could selectively accumulate and kill cancer cell.The interaction between ifosfamide mustards porphyrin dimers and bovine serum albumin(BSA) was investigated by fluorescence spectra.The results showed that the ifosfamide mustards porphyrin dimers own a powerful ability of quenching the fluorescence of BSA via static quenching occurring in the non-covalent porphyrin-BSA complex.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2011年第8期1761-1767,共7页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:J0830415)资助