采用高效液相色谱法同时测定复方丹参滴丸中的多成分含量

Quantitative determination of multi-components in Compound Danshen Dripping Pill by HPLC method

目的:采用高效液相色谱法(HPLC法)同时测定复方丹参滴丸中的7种成分。方法:应用Diamonsil C18色谱柱,以乙腈-甲醇-0.1%磷酸溶液为流动相进行梯度洗脱,在柱温35℃下使用205、270和285 nm三波长同时检测。结果:7种成分的峰面积与浓度的线性关系良好,平均回收率在94.3%～101.3%间,加样回收率符合要求。结论:该方法简单、准确可靠且重现性好,可用于该制剂的质量控制。

Objective： To establish an HPLC method for the simultaneous determination of 7 components in Compound Danshen Dripping Pill.Method： Diamonsil C18 was used as the solid phase,and acetonitrile-methanol-0.1%（v/v） phosphoric acid as the mobile phase for the gradient elution.The column temperature was 35℃,and the detective wavelengths were set as 205,270 and 285 nm.Results： the linearities of the 7 components were all good in the range of the test concentration,and the average recoveries were between 94.3% and 101.3%.Conclusion： the method was simple,reliable and reproducible,which could be used as quality control for the determination of Compound Danshen Dripping Pill.