定性试验与定量试验对杀菌效果评价结果的比较

COMPARISON OF RESULTS OF EVALUATING GERMICIDAL EFFICACY BY QUALITATIVE TEST VERSUS QUANTITATIVE TEST

以干热３００ ℃作用于钢片表面枯草杆菌黑色变种芽胞，以２ ％ 戊二醛溶液作用于布片上的枯草杆菌黑色变种芽胞，以１２１ ±０ ．５ ℃压力蒸汽作用于纸片上嗜热脂肪杆菌芽胞。采用定性与定量试验分别检测杀灭效果。结果，定性试验达到无菌所需作用时间，均较定量试验杀灭率达１００ ％ 者为长。

In orderto comparetheexamination ofgermicidalefficacy by qualitativetestversusquantitativetest,spores of Bacillussubtilisvar. nigeron surface ofsteelcoupon were exposedto 300 ℃ dry heat,spores of B.subtilis var. niger on cloth strip were exposedto 2 % glutaraldehydeso lution and spores of Bacillusstearothermophilus were exposed to 121 ±0.5 ℃autoclaving,fol lowed by synchronousexamination bythetwotests.Theresultsshowedthatthetimerequiredto attain sterilization bythethree germicidalagentsexamined bythequalitativetest waslongerthan thatrequired forattaining 100 % killing rate examined by quantitativetest.