沙蜇Stomopholus meleagris核苷类成分研究

Studies on nucleoside constituents of Jellyfish Stomopholus meleagris

目的首次对沙蜇的核苷类成分进行比较全面的提取、分离和结构鉴定,为沙蜇的进一步综合开发利用提供理论依据。方法新鲜沙蜇的甲醇提取物用石油醚、乙酸乙酯和正丁醇分级萃取;再用硅胶柱层析、Sephadex LH-20凝胶柱层析、薄层制备层析等分离技术得到单体化合物;通过GC-MS、ESIMS、NMR等分析方法,结合文献对照,对所得化合物进行结构鉴定。结果从沙蜇的甲醇提取物中分离出6个单体：胸腺嘧啶（1）、2’-甲氧基尿嘧啶核苷（2）、尿嘧啶（3）、2’-脱氧胸腺嘧啶核苷（4）、2’-脱氧尿嘧啶核苷（5）、尿嘧啶核苷（6）。结论化合物1~6均为首次从沙蜇中分离得到。

Objective For further utilization and exploitation of jellyfish resource,nucleoside constituents from jellyfish Stomopholus meleagris were investigated.Methods Methanol extracts of fresh jellyfish was partitioned successively with petroleum ether,ethyl acetate,and n-butanol.Then silica gel chromatography,Sephadex LH-20 gel filtration and PTLC were performed for the isolation and purification of the compounds,and GC-MS,ESIMS,NMR analyses as well as data comparison with reported literatures were applied for the structure elucidation.Results Six compounds were isolated and determined from jellyfish： thymine（1）,2＇-O-methyluridine（2）,uracil（3）,2＇-deoxythymidine（4）,2＇-deoxyuridine（5）,uridine（6）.Conclusion All the compounds obtained were purified and identified from jellyfish Stomopholus meleagris for the first time.