莲子胚芽油体外抗氧化能力的研究

Study on the antioxidant activities of lotus germ oil in vitro

采用DPPH法和化学发光法对比研究了莲子胚芽油和VE、VC、茶籽油对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)及超氧阴离子(O2-·)、羟自由基(·OH)及过氧化氢(H2O2)等诱导有机体突变的物质的清除作用。结果表明:莲子胚芽油具有清除DPPH自由基、超氧阴离子自由基(O2-·)、羟自由基(·OH)及过氧化氢(H2O2)的作用,表现为中等强度抗氧化活性,半清除率IC50分别48.90、13.48、3.13、0.59mg/mL,明显比茶籽油清除自由基的抗氧化效果好。

The scavenging effects of lotus germ oil comparing to VE,VC and tea seed oil on free radical of 1,1-dippheny-2-picrylhydray radical（DPPH·）,superoxide radical（O-2·）,hydroxyl radical（·OH）and hydrogen peroxide（H2O2）were evaluated by chemiluminescence and DPPH method.The results showed that lotus germ oil had moderate antioxidative activities.Its scavenging concentrations of 50%（IC50）were 48.90,13.48,3.13,0.59mg/mL,respectively.It was better than the antioxidative activities of tea seed oil and it can provide an experimental basis for its further research.