香柏总酚酸提取工艺优化

Optimization of extract processing of polyphenol in Sabina Pingii var.Wilsonii

目的:优化香柏总酚酸的提取工艺。方法:利用回流提取方法进行提取,用七因素二水平正交实验确定影响提取的主要因素,然后用正交回归实验确定主要影响因素的最佳水平。结果:影响香柏总酚酸提取率的因素顺序为:乙醇浓度>液固比>提取时间>回流温度>浸泡时间>pH>提取次数。回流提取的最佳工艺条件:乙醇浓度(V/V)为81.38%,液固比为28.89:1(mL/g),提取时间为37.5min。在最佳工艺条件下,最大提取率可达2.779g/100g。结论:该方法操作简单、方便适用,可以用于工业化生产。

Objective：To optimize the condition of extraction for polyphenol in Sabina Pingii var.Wilsonii.Methods：Using reflux extraction method,the influence factors were studied through orthogonal experiment,and then the extraction technologies were optimized through orthogonal regression design.Results：The factors which affected the extraction rate were ethanol concentrationliquid-solid ratioextraction timereflux temperaturemarination timepHextraction times.The optimum conditions were：ethanol concentration was 81.38%,liquid-solid ratio was 28.89：1（mL/g）,extraction time was 37.5min,the extraction could up to 2.789g/100g under the optimum conditions.Conclusion：This extraction method was simple,rapid and applicable for the extraction of total polyphenol in Sabina pingii var.wilsonii.