脱脂沙棘籽渣中原花青素的提纯与稳定性研究

Separation and stability of proanthocyanidins from nonfat seabuckthorn seed dreg

在单因素实验基础上采用响应面优化法,确定了从脱脂沙棘籽渣中提取原花青素的最佳工艺条件为乙醇体积分数60%,温度35℃,pH1.5,提取时间1h,在此条件下原花青素的提取率为6.61%,纯度为38.63%;同时考察了原花青素粗产品的热稳定性和酸碱稳定性,结果表明,原花青素在温度大于50℃及碱性条件下不稳定,这同时也证明了最优实验条件的合理性;采用溶剂萃取法和AB-8大孔树脂吸附法对原花青素粗产品进行分离提纯,得到产品收率分别为0.5%与1.6%,纯度分别为81.2%与82.8%。

On the base of single factor experiments,the optimum technological conditions of extraction proanthocyanidins from seabuckthorm seed dreg were attained by response surface method.The optimum conditions were as follows：the volume fraction of ethanol was 60%,the temperature of extraction was 35℃,the pH of the solution was 1.5.Under the above conditions,the yield of proanthocyanidins was 6.61% and the purity was 38.63%.The thermal and acid-base stability of proanthocyanidins were also studied.The results showed that proanthocyanidins was unstable above 50℃ and in alkaline conditions,which also elucidated the optimum conditions were reasonable.Solvent extraction and AB-8 macroreticular resins were used to refine procyanidins,the yied of proanthocyanidins was 0.5% and 1.6% respectively while the purity was 81.2% and 82.8%.