摘要
研究了壳聚糖-海藻酸钠内源乳化法制备溶菌酶微胶囊的工艺条件。海藻酸钠浓度2.0%,溶菌酶浓度1.5%,壳聚糖浓度为0.3%,Span80的添加量为油相体积的1.0%时,正交实验得出最佳制备条件为:碳酸钙与海藻酸钠质量比为7:40,水相与油相体积比为30:70,冰醋酸与碳酸钙质量比为1.4:1。然后,研究了溶菌酶微胶囊的粒径分布以及在模拟胃肠液中的控制释放效果和包埋后的溶菌酶在模拟胃液中的稳定性。结果表明,微胶囊粒径大小为483.7μm,粒径分布指数为0.6;优化得到的微胶囊在模拟胃液中2h释放率为35.5%,在模拟肠液4h后总释放率达88.9%;模拟胃液处理后溶菌酶保留活性达90.0%。
The preparation technique of microcapsule from chitosan-alginate sodium was investigated.When the concentration of alginate sodium,chitosan and lysozyme were 2.0%,0.3%,1.5% respectively,and the volume ratio of Span80 to oil was 1.0%,the results from orthogonal experiments were that the mass ration of CaCO3 to alginate sodium was 7:40,and the ratio of water to oil was 30:70,and,and the mass ratio of glacial acetic acid to CaCO3 was 1.4:1.Then the size distribution of microencapsulation and the control-release property of the microcapsule of chitosan-alginate sodium in imitated stomach-liquid environment and the activity of embedded lysozyme in the imitated stomach-liquid were determined.The results showed that the size and SPAN factor of microencapsulation were 483.7μm and 0.6 respectively,the release ratio in imitated stomach-liquid after 2h was 35.5%,and the whole release ratio in imitated liquid after 4h was 88.9%,the activity of embedded lysozyme in the imitated stomach-liquid was 90.0%。
出处
《食品工业科技》
CAS
CSCD
北大核心
2011年第8期318-320,324,共4页
Science and Technology of Food Industry
关键词
海藻酸钠
壳聚糖
内源乳化
溶菌酶
alginate sodium
chitosan
internal gelation
lysozyme