高效液相色谱法同时测定沙枣果实中5种酚类化合物

Simultaneous analysis of five phenolic compounds in fructus elaeagni by high performance liquid chromatography

建立了高效液相色谱法检测沙枣果实中绿原酸、咖啡酸、香豆酸、肉桂酸和阿魏酸等5种酚类物质的方法。样品用乙腈超声提取,色谱柱为HypersilODS100mm×4.6mm(i.d),5μm柱;流动相为乙腈和磷酸缓冲液(0.6%),梯度洗脱;流速为1mL/min;检测波长为323nm和280nm;进样量为10μL。5种组分在一定范围内有良好的线性关系,相关系数为0.991～0.997,平均回收率为93.3%～98.1%,相对标准偏差为3.1%～7.4%(n=6)。结果表明:沙枣果实中绿原酸、咖啡酸、香豆酸、阿魏酸和肉桂酸的含量分别为0.472、0.373、0.425、0.529、0.381mg/kg。

A method had been developed for the simultaneous determination of the contents of chlorogenic acid,caffeic acid,ferulic acid,coumalic acid and cinnamic acid in fructus elaeagni by high performance liquid chromatography.After being extracted by acetonitrile from samples,five phenolic compounds were separated on a Hypersil ODS 100×4.6mm,5μm column using acetonitrile and 0.6% orthophosphoric acid as mobile phase with linear gradient elution,and detected by photodiode array detector（PDAD at wavelength of 323nm and 280nm.There were good linear correlations between the concentrations and peak areas of the five components with the correlation coefficients of 0.991 ~ 0.997.The average recoveries were 93.3% ~ 98.1% with relative standard deviations of 3.1% ~ 7.4%（n=6）.The results suggested that the content of chlorogenic acid,caffeic acid,ferulic acid,coumalic acid and cinnamic acid in fructus elaeagni was 0.472,0.373,0.425,0.529,0.381mg/kg respectively.